Coding

Part:BBa_J62003:Design

Designed by: John Dueber   Group: Keasling Lab   (2008-07-26)

{SH3 ligand}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

four oligonucleotides (two forward and two reverse) designed to anneal to create both the appropriate encoding sequence and 5’ BglII and 3’ XhoI cohesive ends to ligate into BglII/XhoI digested pJD609 (a descendent of pBca9145 with additional multiclonal restriction enzyme sites). In this manner the codons could be optimized and made degenerate. BBa_J62003 was made with oligos JD_H10-H13.

References